SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

A novel mcl-PHA produced on coprah oil by Pseudomonas guezennei biovar. tikehau, isolated from a "kopara" mat of French Polynesia

Simon-colin, Christelle; Alain, Karine; Colin, Sebastien; Cozien, Joelle; Costa, B; Guezennec, Jean; Raguenes, Gerard.

Aims: The aim of the present study was to describe an aerobic, mesophilic and heterotrophic bacterium, designated RA26, able to produce a medium-chain-length polyhydroxyalkanoate (PHA). It was isolated from a French Polynesian bacterial mat located in the atoll of Rangiroa. Methods and Results: This micro-organism, on the basis of the phenotypical features and genotypic investigations can be clearly assigned to the Pseudomonas genus and the name of Pseudomonas guezennei is proposed. Optimal growth occurs between 33 and 37 degrees C, at a pH between 6 4 and 7 1 and at ionic strength of 15 g 1(-1) of sea salts. The G+C content of DNA is 63.2%. Under laboratory conditions, this bacterium produced a novel, medium-chain-length PHA, mainly composed of...

Tipo: Text

Palavras-chave: Pseudomonas; Polyhydroxyalkanaote; Microbial mat; Kopara.

Ano: 2008

URL: http://archimer.ifremer.fr/doc/2008/publication-3858.pdf

Simon-colin, Christelle; Raguenes, Gerard; Crassous, Philippe; Moppert, X; Guezennec, Jean.

Pseudomonasguezennei biovar. tikehau was isolated from a microbial mat on the atoll of Tikehau in French Polynesia, and is able to synthesize medium chain length poly-beta-hydroxyalkanaote copolymers when grown on coprah oil. A two-step cultivation process was used and the biosynthesis of PHAs was followed along 52 h by regular culture sampling. The polyester was purified from freeze-dried cells and analysed by nuclear magnetic resonance (NMR), Fourier transform infra red (FTIR), and gas chromatography mass spectrometries. The copolyester produced by P. guezennei biovar. tikehau from coprah oil mainly consisted of saturated monomers, i.e. 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate (3HD), and the monomeric composition of the polyester did not change...

Tipo: Text

Palavras-chave: Polyhydroxyalkanaote; PHAs; Pseudomonas; "kopara"; Microbial mat.

Ano: 2008

URL: http://archimer.ifremer.fr/doc/2008/publication-6119.pdf

Ação do imazapic+imazapyr sobre a tiririca (Cyperus rotundus) e os desnitrificadores em milho

Planta Daninha

Ulbrich,A.V.; Leite,C.R.F.; Souza,J.R.P.; Andrade,D.S..

Os objetivos do experimento foram avaliar a eficácia do imazapic+imazapyr sobre a tiririca e a influência desta planta daninha sobre a população de desnitrificadores em solo cultivado com milho. O experimento foi realizado em caixas de 0,70 x 0,30 x 0,30 m, onde foram semeados o milho tolerante às imidazolinonas (C-901CL) e plantados 50 bulbos de tiririca. O delineamento experimental foi o inteiramente casualizado, com seis repetições. Os tratamentos foram constituídos por: 1. milho sem tiririca; 2. milho com tiririca; 3. milho com tiririca capinada; 4. milho com tiririca e imazapic+imazapyr (63+21g ha-1 de i.a.) em pós-emergência; e 5. milho com tiririca e imazapic+imazapyr (63+21g ha-1 de i.a.) em pré-emergência. O herbicida foi eficiente em...

Bacterial Community Associated with Fish and Water from Congonhas River, Sertaneja, Paraná, Brazil

Palavras-chave: Bactéria; Pseudomonas; Nitrogênio; Volatilização; Competição.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582004000400012

BABT

Sousa,José Américo de; Silva-Souza,Ângela Teresa.

A bacteriological study was conducted on fish and water from Congonhas River, Sertaneja (22º58 S; 50º58 W), Paraná State, Brazil. From 44% of the analysed fish, bacteria belonging to Pseudomonas, Acinetobacter, Aeromonas, Enterobacteriaceae, Micrococcus, Bacillus and Lactobacillus were isolated. The group most frequently isolated from fish was Aeromonas. In the water, the bacterial groups detected were Pseudomonas, Acinetobacter, Aeromonas, Enterobacteriaceae, Bacillus and Flavobacterium, from which Flavobacterium and Acinetobacter were the most abundant. The numbers of Colony Forming Units per millilitre of water varied from 3.1x10² to 1.0 x 10³. Although a clear pattern was not detected in the susceptibilities/resistances of the isolated strains to...

Bacteries associees aux production de Brachionus plicatilis

Palavras-chave: Bacterial community; Fish; Congonhas River; Aeromonas; Pseudomonas; Resistance.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000400007

Nicolas, Jean-louis; Joubert, M.

Bacteria associated with semi-continuous culture of Brachionus plicatilis , which is used as the first food of marine fish larvae, were counted on Zobell 2216 E medium and identified. The numbers of bacteria were very high in both Brachionus (about 10 super(11) bact. per ml of digestive content) and in water (from 1.6.10 super(6) to 4.7.10 super(7) bact. per ml). The cultures were studied at driving a time when mortalities occurred frequently. The introduction of a Pseudomonas) phenon at a proportion of about 50% to the dominant microflora persisted in time and was little influenced by diet. It has been the cause of the repression of the Vibrio, Aeromonas group which was found in an unusually small proportion. It induced a lack of microflora diversity and...

Tipo: Text

Palavras-chave: Brachionus plicatilis; Rotifera; Pseudomonas; Aeromonas; Vibrio; Nutrition; Pathology; Fish culture; Fish larvae; Food organisms; Bacteria; Continuous culture; Ecological associations; Zooplankton culture.

Ano: 1984

URL: http://archimer.ifremer.fr/doc/1984/acte-994.pdf

Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium

Santisi,Santina; Cappello,Simone; Catalfamo,Maurizio; Mancini,Giuseppe; Hassanshahian,Mehdi; Genovese,Lucrezia; Giuliano,Laura; Yakimov,Michail M..

Three bacterial isolates identified as Alcanivorax borkumensis SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeri SDM, based on 16S rRNA gene sequences, were isolated from crude oil enrichments of natural seawater. Single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (Alcanivorax: Pseudomonas, 1:1), (Alcanivorax: Rhodococcus, 1:1), (Pseudomonas: Rhodococcus, 1:1), and (Alcanivorax: Pseudomonas: Rhodococcus, 1:1:1), were analyzed in order to evaluate their oil degrading capability. All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source). Measures of total and live...

Biofilms: microbes and disease

Palavras-chave: Alcanivorax; Pseudomonas; Rhodococcus; Bioremediation; Bioaugmentation.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200377

BJID

Aparna,Madhu Sharma; Yadav,Sarita.

Bacteria that attach to surface aggregate in a hydrated polymeric matrix of their own synthesis to form biofilms. These represent microbial societies with their own defense and communication system. Transitioning from acute to chronic infection is frequently associated with biofilm formation.Bacteria in biofilms are innately more resistant to antimicrobial agents. The presence of indwelling medical devices increases the risk for biofilm formation and subsequent infection. The current antibiotic therapies are of limited effectiveness in resolving biofilms infection.This review attempts to discuss the stages in biofilm formation, their pathogenic mechanisms, effect of antimicrobial agents, detection and eradication of the biofilms.

Biological control of potato black scurf by rhizosphere associated bacteria

Palavras-chave: Biofilms; Staphylococci; Pseudomonas; Candida.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600016

Tariq,Mohsin; Yasmin,Sumera; Hafeez,Fauzia Y..

The present work was carried out to study the potential of plant rhizosphere associated bacteria for the biocontrol of potato black scurf disease caused by Rhizoctonia solani Khun AG-3. A total of twenty-eight bacteria isolated from diseased and healthy potato plants grown in the soil of Naran and Faisalabad, Pakistan were evaluated for their antagonistic potential. Nine bacterial strains were found to be antagonistic in vitro, reduced the fungal growth and caused the lysis of sclerotia of R. solani in dual culture assay as well as in extracellular metabolite efficacy test. The selected antagonistic strains were further tested for the production and efficacy of volatile and diffusible antibiotics, lytic enzymes and siderophores against R. solani. Selected...

Carbazole degradation in the soil microcosm by tropical bacterial strains

Palavras-chave: Biological control; Potato black scurf; Rhizobacteria; Pseudomonas; Rhizoctonia solani.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200026

Salam,Lateef B.; Ilori,Matthew O.; Amund,Olukayode O..

In a previous study, three bacterial strains isolated from tropical hydrocarbon-contaminated soils and phylogenetically identified as Achromobacter sp. strain SL1, Pseudomonas sp. strain SL4 and Microbacterium esteraromaticum strain SL6 displayed angular dioxygenation and mineralization of carbazole in batch cultures. In this study, the ability of these isolates to survive and enhance carbazole degradation in soil were tested in field-moist microcosms. Strain SL4 had the highest survival rate (1.8 x 107 cfu/g) after 30 days of incubation in sterilized soil, while there was a decrease in population density in native (unsterilized) soil when compared with the initial population. Gas chromatographic analysis after 30 days of incubation showed that in...

Catabolism of caffeine and purification of a xanthine oxidade responsible for methyluric acids production in Pseudomonas putida L

Palavras-chave: Bioaugmentation; Biodegradation; Microcosm; Pseudomonas; Microbacterium; Achromobacter; Carbazole.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401037

Rev. Microbiol.

Yamaoka-Yano,Dirce Mithico; Mazzafera,Paulo.

Caffeine catabolism and a xanthine oxidase involved in the alkaloid breakdown were studied in Pseudomonas putida L, a strain displaying high ability to grow on this substrate. Cells cultured with unlabelled caffeine and 14C labeled caffeine and xanthine showed that this alkaloid was broken-down via theobromine/paraxanthine -> 7-methylxanthine -> xanthine -> uric acid -> allantoin -> allantoic acid. Methyluric acids were formed from the oxidation of theobromine, paraxanthine and 7-methylxanthine, although no bacterial growth was observed on these compounds, indicating that this might be due to a wide substrate specificity of xanthine oxidase. This was confirmed by activity staining in PAGE where activity was observed with...

Characterization of the spoilage potential of pure and mixed cultures of bacterial species isolated from tropical yellowfin tuna ( Thunnus albacares )

Palavras-chave: Caffeine; Methylpurines; Methyluric acids; Pseudomonas; Xanthine oxidase.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100013

CONTROL DE LA FUSARIOSIS VASCULAR DEL GARBANZO (Cicer arietinum L.) POR MICROORGANISMOS NATIVOS DE SINALOA, MÉXICO

Silbande, A.; Cornet, Josiane; Cardinal, Mireille; Chevalier, Frederique; Rochefort, K.; Smith-ravin, J.; Adenet, S.; Leroi, Francoise.

Aim The spoilage potential of 28 bacterial strains isolated from spoiled raw yellowfin tuna was evaluated. Methods and Results Bacterial species were inoculated in irradiated tuna matrix. Chemical changes, bacterial growth and sensory quality were monitored during aerobic storage at 8°C. Pseudomonas spp., Enterobacter spp. and Escherichia hermanii had no spoiling effect. Brochothrix thermosphacta and Carnobacterium divergens/maltaromaticum developed moderate unpleasant odors. Hafnia paralvei and Serratia spp. released strong off-odors (pyrrolidine, sulfur/cabbage). No bacterial group (except H. paralvei) combined with Pseudomonas spp. deteriorated the sensory quality of tuna. When C. divergens/maltaromaticum was associated with H. paralvei or B....

Tipo: Text

Palavras-chave: Bacterial interactions; Bacterial species; Fish; Pseudomonas; Sensory quality; Spoilage potential; Tropical; Tuna.

Ano: 2018

URL: https://archimer.ifremer.fr/doc/00414/52557/53395.pdf

Agrociencia

Oliva-Ortiz,Luz del C.; Velázquez-Alcaraz,Teresa de J.; Sosa-Pérez,Rogelio; Partida-Ruvalcaba,Leopoldo; Díaz-Valdés,Tomás; Arciniega-Ramos,Julio; López-Orona,Carlos A..

Resumen La fusariosis vascular del garbanzo (Cicer arietinum L.) aumenta debido a la dificultad para controlarla. El control químico disminuye la biodiversidad y contamina el ambiente, por lo cual se deben desarrollar medidas sustentables para limitar la enfermedad. Por lo tanto, el objetivo de este estudio fue determinar el potencial de cepas bacterianas y fúngicas, nativas de Sinaloa, como agentes biocontrol de esta enfermedad en plantas de garbanzo cultivadas en invernadero. La hipótesis fue que las cepas bacterianas y fúngicas nativas son una alternativa para controlar de la fusariosis vascular del garbanzo porque inhiben Fusarium oxysporum f. sp. ciceris. La inhibición de las cepas de control biológico se evaluó sobre el crecimiento radial de Fusarium...

URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952017000600683

Palavras-chave: Control biológico; Cepas nativas; Bacillus; Trichoderma; Pseudomonas; Fusarium oxysporum f. sp. ciceris.

Ano: 2017

Degradation of caffeine by microorganisms and potential use of decaffeinated coffee husk and pulp in animal feeding

Scientia Agricola

Mazzafera,Paulo.

Coffee husk and coffee pulp are coffee processing by-products. Coffee husk is obtained when harvested coffee is processed by the dry method, and coffee pulp is produced by the wet method. In Brazil, coffee is usually processed by the dry method, therefore an expressive amount of husk is obtained every year. Some of the husk is used as organic fertilizer but, other applications are very limited, mainly because it is a bulky product. The presence of tannins and caffeine diminish acceptability and palatability of husk by animals. This review discuss degradation of caffeine by microorganisms, with special attention to bacterial, biological decaffeination of coffee husk and pulp and its subsequent use on animal feeding. The known biochemical routes of caffeine...

Detecção de bacteriocinas produzidas por bactérias fitopatogênicas dos gêneros Erwinia, Pseudomonas e Xanthomonas

Palavras-chave: Pseudomonas; Coffee husk; Decaffeination.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000400030

Scientia Agricola

Biagi,C.M.R. de; Azevedo,J.L. de.

A detecção da produção de bacteriocinas foi estudada em diferentes condições, utilizando-se linhagens de bactérias fitopatogênicas dos gêneros Erwinia, Pseudomonas e Xanthomonas. Obtiveram-se, respectivamente, 58,06%, 71,31% e 40,00% de linhagens produtoras no meio 523, que foi o mais indicado para detecção dessa produção. Uma maior concentração de ágar adicionada ao meio (1,5%) produziu melhores resultados mas, a quantidade de meio nas placas não influenciou a detecção de bacteriocinas. O maior tempo de incubação utilizado (72 h.) facilitou detecção de halos de produção de bacteriocinas. Irradiação com luz ultra-violeta em baixas doses parece facilitar a liberação de bacteriocinas, mas isso varia com a linhagem empregada.

Diversidad y caracterización de poblaciones bacterianas asociadas a esclerocios de Claviceps gigantea Fuentes et al. en maíz (Zea mays L.) con potencial antagonista, en el Estado de México.

Palavras-chave: Bacteriocinas; Xanthomonas; Pseudomonas; Erwinia.

Ano: 1992

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161992000400002

Colegio de Postgraduados

Ayala Torres, Ana María.

Un total de 129 aislamientos bacterianos obtenidos de la micósfera de esclerocios de Claviceps gigantea se evaluaron in vitro para determinar su capacidad de inhibir el crecimiento de Fusarium graminearum, F. subglutinans y F. verticillioides patógenos a maíz. Ensayos cualitativos en confrontación dual, resultaron en la selección de 22 aislamientos bacterianos con el mayor potencial antagónico hacia una, dos o las tres especies de Fusarium. Los aislamientos se identificaron molecularmente mediante secuenciación parcial del gen 16s ADNr. Las cepas con mayor grado de antagonismo pertenecen a los géneros Bacillus y Pseudomonas. La caracterización en la producción de metabolitos potencialmente involucrados en la actividad antagónica, mostró que el 100% de las...

Palavras-chave: Bacillus; Pseudomonas; Biocontrol; Metabolitos secundarios; Caracterización; Secondary metabolites; Characterization; Fitopatología; Maestría.

Ano: 2014

URL: http://hdl.handle.net/10521/2299

Effectiveness of halo-tolerant, auxin producing Pseudomonas and Rhizobium strains to improve osmotic stress tolerance in mung bean (Vigna radiata L.)

Ahmad,Maqshoof; Zahir,Zahir A.; Nazli,Farheen; Akram,Fareeha; Arshad,Muhammad; Khalid,Muhammad.

Halo-tolerant, auxin producing bacteria could be used to induce salt tolerance in plants. A number of Rhizobium and auxin producing rhizobacterial strains were assessed for their ability to tolerate salt stress by conducting osmoadaptation assay. The selected strains were further screened for their ability to induce osmotic stress tolerance in mung bean seedlings under salt-stressed axenic conditions in growth pouch/jar trials. Three most effective strains of Rhizobium and Pseudomonas containing ACC-deaminase were evaluated in combination, for their ability to induce osmotic stress tolerance in mung bean at original, 4, and 6 dS m-1 under axenic conditions. Results showed that sole inoculation of Rhizobium and Pseudomonas strains improved the total dry...

Effects of Brassica napus seed meal amendment on soil populations of resident bacteria and Naegleria americana, and the unsuitability of arachidonic acid as a protozoan-specific marker

Palavras-chave: ACC-deaminase; Pseudomonas; Rhizobium; Osmotic stress; Salt tolerance index.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400045

OAK

Cohen, Michael F.; Mazzola, Mark.

Bacterial and protozoan populations were monitored in orchard soil amended with Brassica napus seed meal. Numbers of fluorescent pseudomonads peaked one week following incorporation of the amendment and declined thereafter, whereas populations of Streptomyces spp. increased to stable levels nearly 20-fold higher than in non-treated soil. The amoeba-flagellate Naegleria americana, isolated from the amended soil, showed a marked feeding preference in culture for fluorescent pseudomonads relative to Streptomyces spp. Amended soils showed rapid declines in RSM-derived cis-oleic acid that paralleled increases in the numbers of bacteria and proportion of bacterial fatty acid markers in the soil. Arachidonic acid, which reached peak levels one week after seed...

Palavras-chave: Orchard soil; Protozoan feeding; Streptomyces; Pseudomonas; Fatty acid markers.

Ano: 2006

URL: http://ir.obihiro.ac.jp/dspace/handle/10322/143

Eliminação da produção de bacteriocinas em Erwinia e Pseudomonas fitopatogênicas

Scientia Agricola

Biagi,C.M.R. de; Azevedo,J.L..

Duas linhagens de bactérias fitopatogênicas do gênero Pseudomonas e duas do gênero Erwinia produtoras de bacteriocinas, foram submetidas a diferentes tratamentos visando estimar a estabilidade desse caráter. Houve eliminação da produção de bacteriocinas após tratamento com duas concentrações de brometo de etídio e a porcentagem de eliminação variou com o hospedeiro e com a concentração da droga. Não houve eliminação em temperaturas elevadas e também a preservação da linhagem por três anos em laboratório não causou perda do caráter. A eliminação da produção de bacteriocinas com brometo de etídio sugere que os genes envolvidos tenham localização plasmidiana.

Evaluation and biochemical characterization of a distinctive pyoverdin from a pseudomonas isolated from chickpea rhizosphere

Palavras-chave: Bacteriocina; Erwinia; Pseudomonas.

Ano: 1995

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161995000100024

Tank,Neelam; Rajendran,Narayanan; Patel,Baldev; Saraf,Meenu.

Microbial siderophores confiscate the available ferric ions around the roots and trigger a reaction resulting in plant growth promotion. In our study, a high level of siderophore production was observed from a newly isolated Pseudomonas sp. from the rhizosphere of Chickpea plants. Under an iron depleted condition in Standard Succinic acid medium a 1000 µgmL-1 of siderophore production was achieved. Increasing the concentration of iron showed an inverse relationship between growth and siderophore production. Fourier Transform Infrared Spectroscopy (FTIR) analysis of the purified crystals, its UV spectral analysis and High Pressure Liquid Chromatography (HPLC) revealed the identity of the siderophore as similar to that of pyoverdin with distinctive...